|
KMID : 0371020020350040269
|
|
Journal of Preventive Medicine and Public Health
2002 Volume.35 No. 4 p.269 ~ p.274
|
|
|
Effect of Glutathione on Lead Induced Modulation of NO Synthesis in RAW 264.7 Cell
|
|
Shin Sae-Ron
Oh Gyung-Jae
Kwon Keun-Sang
Yoon Wook-Hee
|
|
|
Abstract
|
|
|
Objectives: To evaluate the effect of glutathione(GSH) on lead induced modulation of nitric oxide(NO) synthesis, and to examine how lead modulates NO production in macrophages.
Methods: This study was observed in a culture of RAW 264.7 cells, which originated from a tumor in a Balb/c mouse that was induced by the Abelson murine leukemia virus. The compounds investigated were lead chloride, N-acetyl-cystein(NAC),
and Buthionine Sulfoximine(BSO).
Results: ATP synthesis in RAW 264.7 cells was unchanged by each lead concentration exposure in a dose dependent manner. The NO synthesis was decreased when exposed to lead(PbCl2) concentration 0.5 ㎛. The presence of 300 ㎛ NAC, used as a pretreatment in the culture medium, caused the recovery of the lead induced decrease in NO synthesis, but in the presence of 300 ㎛ BSO as a pretreatment, there was no recovery. Pretreatment with NAC and BSO had no affect on ATP synthesis at any on the lead concentrations used.
Conclusions: These results indicated that GSH has a protective effect toward lead toxicity, and suggested that the inhibition of NO production in macrophage due to lead toxicity may be related to cofactors of iNOS (inducible nitric oxide synthase)
|
|
|
KEYWORD
|
|
|
Glutathione, Lead, Buthionine sulfoximine, Nacetycysteine, Nitric oxide
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|